immunology & Biochemistry
mozhgan naderi kotaki; azizeh asadzadeh; fatemeh heidaryan
Volume 27, Issue 5 , November and December 2020, , Pages 594-602
Abstract
Introduction: Alzheimer’s disease is the most common cause of dementia in the elderly and is characterized by loss of memory and other cognitive functions. The acetylcholinesterase enzyme (ACHE) with hydrolysis of the neurotransmitter acetylcholine in the cholinergic synapses of the brain plays ...
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Introduction: Alzheimer’s disease is the most common cause of dementia in the elderly and is characterized by loss of memory and other cognitive functions. The acetylcholinesterase enzyme (ACHE) with hydrolysis of the neurotransmitter acetylcholine in the cholinergic synapses of the brain plays a role in ending the neural message. One of the methods used to control the progression of Alzheimer’s disease is the administration of cholinesterase inhibitors, especially plant- originated medicines. The aim of this study was to study bioinformatics and obtain the level of inhibitory effect of acetylcholinesterase enzyme by hydro-alcoholic extract of aerial organs of Thymus vulgaris. Materials and Methods: In order to investigate how the compounds are attached to the active site of the enzyme, first, Docking studies were conducted using AutoDock 4.2 software, the hydro-alcoholic extract of Thymus vulgaris was measured at concentrations 2, 1, 0 .5, 0.25 mg/ml in order to determine its inhibitory effect on acetylcholinesterase. Results: The compounds present in the extract were able to occupy the active site of the enzyme with a binding energy level of -4.12 to -6.58 kcal / mol. According to the laboratory results, concentrations of 2mg/ml were 46.99% and IC50 = 2.03 mg/ml. Conclusion: The results of in silico and in vitro studies indicate that the extract of this plant contains effective inhibitory compounds, and trying to use these compounds is a suitable topic for future research with the aim of acquiring pharmacologically inhibitors.